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Equipment Required
The ArrayTube™ reader is a rapid, compact, single tube reading device, which enables the capture and visualisation of the array image. It comes with a USB interface and is easily connected to a PC or laptop.
The ArrayMate™ reader provides a higher throughput of samples by reading up to 6 ArrayTubes or 12 ArrayStrips at one time. Each ArrayStrip comprises 8 wells, with the potential for 600 probes in the base of each well.
The software is a powerful imaging tool for the analysis of array images of multiple formats and ensures a high level of data integrity.
All other equipment required is standard in most molecular biology laboratories, including PCR machine, incubator, thermomixer and pipettes.
Assay Principle
Bacterial and viral genotyping on the spot!
Identibac is a simple, high-throughput system of DNA microarrays for the simultaneous detection of multiple genes in bacteria and viruses including subtyping, antimicrobial resistance genes, toxin- and virulence genes. This genetic fingerprinting can support infection control in hospitals, communities and reference labs. Rapid strain identification helps to trace outbreaks or epidemiological changes without sequencing.
Assay principle is described for bacterial Genotyping (for S. aureus, E. coli and AMR -ve)
Following the extraction of genomic DNA the technique has four basic stages:
Amplification
Extracted DNA is labelled with Biotin (L) and amplified in a linear multiplex PCR reaction.
Hybridization
The Biotin labelled, single-stranded PCR product hybridizes to the corresponding probes. The bound PCR product is detected using a horse-radish peroxidase (E) – streptavidin conjugate, which converts the substrate [seramun green] (S) into a coloured precipitate.
Detection
The Reader (ArrayMate™ or ATR 03 Reader) enables the capture and visualisation of the array image. The presence of a dark spot indicates successful hybridization.
Analysis
The software, supplied with the ArrayMate™ Reader (or ArrayTube™ Reader ATR 03), measures the signal intensity of each probe and determines which genes are present in the sample.